Reactions of excess Big Dye Terminator dyes, and run the reactions on the sequencer.
#Sequence alignment 4peaks full
This full service option is suggested when you have less than 15 samples to sequence. Electrophoresis only service: Your laboratory runs the sequencing reactionsĪnd removes excess dye.Template and primer requirements for Reaction & Electrophoresis service 4PEAKS SEQUENCE ALIGNMENT SOFTWARE FULL High-Throughput sequencing service: Your sample and primer are submittedĪs a mixture for each sequencing reaction in a 96-well sequencer compatible plate.Requirements for Electrophoresis only service The Genomics Core runs your reactions on the sequencer. Provide 10 ul of the template for each reaction desired.Samples and primer must be dissolved in distilled water.Fragment Analysis: You submit fluorescent-labeled PCR fragments for analysis.Requirements for High-Throughput sequencing service The Genomics Core adds the sequencing reagents, cycles the plate, cleans the excessĭye from the reactions and runs the plate on the sequencer. One template/primer set equals one sequencing reaction. The templates should be placed in column order as listed If you are submitting 20 or more samples, the template must be provided in a standard.Two primers equals two sequencing reactions, etc. 4PEAKS SEQUENCE ALIGNMENT SOFTWARE FULL.
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